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In recent years, electronic nicotine delivery systems (ENDS), commonly known as electronic cigarettes (ECs), have gained popularity, particularly among adolescents (Cooper, 2022). • The core component of these devices is e-liquids which contain nicotine, propylene glycol, vegetable glycerin, flavoring, and other chemicals (Cheng, 2014; Sassano, et al., 2018). • The FDA regulates manufacturing, importing, packaging, labelling, advertising, promotion, sale, and distribution of ENDS, but they do not check the accuracy of reported information. • Studies have consistently shown that labelled nicotine concentration and actual concentration can range from as little as 35% less nicotine to as much as 52% greater nicotine (Raymond, et al., 2018; Alhusban & Ata, 2020). • Due to the potential variation in nicotine concentration, it is vital to establish a preparation technique that limits variation in nicotine concentration. • In the present study we utilized mass preparation as a method to limit variation in nicotine preparation. We are comparing two different types of analysis, HPLC vs. GCMS, to ensure method accuracy. • This study is the first in a series investigating variation in nicotine concentration results and the next study we will be investigating the potential impact of flavoring on the concentration of nicotine detected in ENDS and ECs.

Publication Date

4-16-2025

Disciplines

Higher Education | Scholarship of Teaching and Learning

Quantification of Nicotine using High-Performance Liquid Chromatography & Gas Chromatography-Mass Spectrometry

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