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Nicotine is a highly addictive chemical present in the tobacco plant. E-cigarettes, predominantly used among the adolescent population, are becoming increasingly popular. We can quantify the nicotine levels in unknown solutions using HPLC (high-performance liquid chromatography) by first preparing a calibration curve from standard nicotine samples. This experiment aims to understand the difference between using various solvents for the nicotine samples and how using one solvent may be more effective.

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Comparison of Methanol, Water, and HPLC Mobile Phase as the Solvent for Quantification of Nicotine Using HPLC Method



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